书目名称 | Nitric Oxide Protocols | 编辑 | Aviv Hassid | 视频video | | 概述 | Includes supplementary material: | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each | 出版日期 | Book 2004Latest edition | 版次 | 2 | doi | https://doi.org/10.1385/1592598072 | isbn_softcover | 978-1-61737-443-2 | isbn_ebook | 978-1-59259-807-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2004 |
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