Titlebook: Next Generation Sequencing; Methods and Protocol Steven R. Head,Phillip Ordoukhanian,Daniel R. Salo Book 2018 Springer Science+Business Med

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书目名称Next Generation Sequencing影响因子(影响力)




书目名称Next Generation Sequencing影响因子(影响力)学科排名




书目名称Next Generation Sequencing网络公开度




书目名称Next Generation Sequencing网络公开度学科排名




书目名称Next Generation Sequencing被引频次




书目名称Next Generation Sequencing被引频次学科排名




书目名称Next Generation Sequencing年度引用




书目名称Next Generation Sequencing年度引用学科排名




书目名称Next Generation Sequencing读者反馈




书目名称Next Generation Sequencing读者反馈学科排名

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Custodian

ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Ctrates how robust, bio-orthogonal chemistry can be harnessed in vitro to capture and dissect complex biological processes. Here, we describe an updated protocol for the synthesis of “ClickSeq” libraries.

易怒

A Bloody Primer: Analysis of RNA-Seq from Tissue Admixtures,opment of a means of estimating the cell type composition of blood samples from their bulk RNA-Seq profiles. We also illustrate the importance of adjusting for the potential confounding effect of cellular heterogeneity in the context of statistical inference in a whole blood RNA-Seq dataset.

Esophagus

Next-Generation Sequencing of Genome-Wide CRISPR Screens,ens using high-throughput next-generation sequencing (NGS) allows for the adoption of the technology in a variety of laboratories interested in diverse biologic questions. Here, we describe the protocol to generate next-generation sequencing libraries from genome-wide CRISPR genomic screens.

憎恶

An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome,ing purified B cells. We standardized our protocols to directly compare the results from these two approaches. Parallel assessment of translatome with these two approaches can generate a comprehensive picture on how translational regulation determines protein output.

jettison

Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis,hole genome amplification (WGA) on isolated blastomere(s), up to data analysis for CNA detection. The process is described generically and can also be used to perform CNA analysis on a limited number of cells (down to a single cell) in other applications. This unique description also includes some tips and tricks to increase the chance of success.

EVEN

不公开

Primer Extension, Capture, and On-Bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Dg, nascent RNA sequencing, and other protocols where polymerase termination sites need to be profiled. The utilization of solid-phase bead chemistries facilitates simple workflow and efficient library yields.

屈尊

Genome-Wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Apprpre-amplified with concomitant integration of the sequencing adapters, and libraries are subsequently amplified and indexed by PCR. Using scBS-Seq we can accurately measure DNA methylation at up to 50% of individual CpG sites and 70% of CpG islands.