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Titlebook: New Developments and New Applications in Animal Cell Technology; Proceedings of the 1 Otto-Wilhelm Merten,Pierre Perrin,Bryan Griffiths Con

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Expression of Recombinant Human Insulin in Chinese Hamster Ovary Cells is Complicated by Intracellul of insulin degrading enzyme (IDE). No noticeable effects were seen with the addition of a general protease inhibitor, PMSF. When transfected CHO cultures were supplemented with small quantities of bacitracin insulin expressionimproved considerably. These results combined support the hypothesis that
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Transient Transfection in Mammalian Cellsene expression was studied in labscale as well as in fermentor scale by combining DOM with PEI to form transfection complexes..Our data show the PEI-mediated transfection into EBNA cells can be used as a cost-effective transfection system in spinner culture scale up to 2-5L, to produce milligram amo
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Luciano. Kamos,James F. Kane,Amy A. Murnanesceptible to the applied herbicide nonetheless gave PCR data that indicated presence of the gene responsible for herbicide resistance. Similarly, “DNA silencing” of the gene of interest may be investigated if the seeds can be screened and scored for that phenotypic trait in a nondestructive manner prior to planting.
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