Titlebook: Neutrophil Methods and Protocols; Mark T. Quinn,Frank R. DeLeo Book 2014Latest edition Springer Science+Business Media, LLC 2014 NADPH oxi

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Measurement of Phospholipid Metabolism in Intact Neutrophilsfor assessing the activities of phospholipase A. (PLA.), phospholipase C (PLC), phospholipase D (PLD), and phosphoinositide 3-OH-kinase in intact neutrophils. PLA. activity is measured as the release of radiolabeled arachidonic acid. PLC activity is measured as the accumulation of inositol 1,4,5-tri

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Optical Methods for the Measurement and Manipulation of Cytosolic Calcium Signals in Neutrophilsrate Ca. signals and the cellular responses triggered by them. Optical methods for this are the most applicable for neutrophils and are discussed here, especially the use of fluorescent indicators of Ca. and photoactivation of reagents involved in Ca. signaling. Both of these synthetic agents can be

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牙齿

Assessment of Neutrophil Apoptosisammed cell death by apoptosis occurs with maintenance of an intact cell membrane in order to prevent the release of histotoxic intracellular products such as proteases and reactive oxidant species into the extracellular surroundings as occurs during necrosis. Macrophage phagocytosis results in atten

歹徒

Microinjection Methods for Neutrophilslso of monitoring changes using indicators which otherwise cannot be introduced into the cell. However, neutrophils cannot be microinjected by the conventional glass pipette insertion method. Here, we outline two techniques which work well with neutrophils, namely, SLAM (simple lipid-assisted microi

Buttress

Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cellsture cell lineage under the appropriate conditions allows for modeling of cell processes as well as disease states. Here, we describe an in vitro method for generating functional mature neutrophils from human iPSCs. We also describe assays for testing these differentiated cells for neutrophil charac

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Neutrophil Migration Through Extracellular Matrixng encountered, different modes of movement. Neutrophil locomotion can range from mesenchymal to amoeboid movement and may include multiple shape changes, contractile squeezing through gaps, and adhesion/de-adhesion cycles. In vitro migration assays reflect only some aspects of the complex in vivo n

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Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafishtors. The spinning disk confocal system is capable of generating images of fluorescent live samples with broad dynamic range and high temporal and spatial resolution. The ability to acquire fluorescent images of living cells in vivo on a millisecond timescale allows the dissection of biological proc

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