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Titlebook: Neurotrophin Protocols; Robert A. Rush Book 2001 Springer Science+Business Media New York 2001

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发表于 2025-3-21 18:08:21 | 显示全部楼层 |阅读模式
书目名称Neurotrophin Protocols
编辑Robert A. Rush
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Neurotrophin Protocols;  Robert A. Rush Book 2001 Springer Science+Business Media New York 2001
描述The past decade has seen an extraordinary growth in research interest in neurotrophic factors, and the study of the neurotrophin family has led this activity. Nevertheless, this area of research has often struggled as a result of techniques that were either inadequate or just emerging from other research fields and disciplines. Neurotrophin Protocols has brought together many leaders in the neurotrophin field who detail their special expertise in a wide variety of techniques. Though most procedures are valid across many diff- ent fields of research, some of those described here have been developed to address particular issues within the neurotrophic factor field. The protocols cover a broad range of biochemical, histological, and biological techniques that are often required by the modern laboratory. However, all have been written with sufficient detail to allow any laboratory to achieve proficiency without need of reference to other texts. Neurotrophin Protocols is divided into four sections dealing with p- tein, RNA, recombinant, and in vivo techniques. Protein techniques have in general been less successfully employed than those dealing with RNA or DNA. However, procedures that
出版日期Book 2001
版次1
doihttps://doi.org/10.1385/1592590608
isbn_softcover978-1-4899-3983-8
isbn_ebook978-1-59259-060-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2001
The information of publication is updating

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Localization of Neurotrophin Proteins Within the Central Nervous System by Immunohistochemistryeaching an understanding of endogenous neurotrophin function. One technique for addressing many of these issues is immunohistochemistry. The strength of the immunohistochemical approach lies with its ability to precisely define the distribution of a given protein within a tissue sample obtained unde
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Neurotrophin Immunohistochemistry in Peripheral Tissuesal intervention. The movement of the neurotrophins has been studied extensively by the use of nerve ligations, which allows determination not only of the retrograde and/or anterograde movement of the protein, but also can be useful in demonstrating the absence of a neurotrophin in a particular class
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Quantification of mRNA Levels Using Ribonuclease Protection Assaym of a sequence that encodes a portion of neurotrophin mRNA. This DNA fragment can either be part of a cDNA clone or a genomic subfragment, but it should be selected so that the plasmid construct can be digested with a specific restriction enzyme prior to transcription, to yield a labeled RNA specie
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Quantification of Neurotrophin mRNA by RT-PCRrsatility, where total RNA is first reverse transcribed and then amplified by PCR reaction (.,.). The technique can be used to detect the presence or absence of gene transcripts, to measure expression levels semiquantitatively for comparison of mRNA levels in different tissues, or to determine the a
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,,oles of neurotrophins are often closely related to their gene expression during development. Detection of their mRNA expression in developing and in experimental animals has accumulated considerable data revealing many biological functions of these molecules in the normal development and in patholog
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Construction of Cells Expressing Neurotrophinsarge precursor protein within which mature, bioactive NGF occupies the C-terminal half, and the N-terminal half contains a signal peptide (18 amino acid) preceding a large pro region. The pro sequence in combination with the signal (pre) sequence is necessary for the secretion of correctly processed
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