书目名称 | Neurotrophic Factors | 副标题 | Methods and Protocol | 编辑 | Stephen D. Skaper | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Nervous system development evolves from the well-orchestrated processes of neural induction, cell proliferation, differentiation, cell migration, survival, and synapse formation. Among these environmental cues, neurotrophic factors are secreted proteins that promote neurite outgrowth, neuronal cell differentiation and survival both .in vivo. and .in vitro.. Nerve growth factor (NGF) is the founding and best characterized member of the neurotrophin family of neurotrophic polypeptides. .Neurotrophic Factors: Methods and Protocols. presents a selection of protocols and procedures which make use of cellular, tissue, and whole animal models which can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. Chapters cover a wide-range of topics such as dealing with the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the evaluation of neuroprotective molecules, as well as assays which can be applied to the study of agents with neuroregenerative potential. Protocols describing viral- and nanoparticle-based delivery methods to neural cells are also presented, fo | 出版日期 | Book 2012 | 关键词 | nerve growth factor (NGF); neural cells; neurite outgrowth; neurobiological research; neuronal cell diff | 版次 | 1 | doi | https://doi.org/10.1007/978-1-61779-536-7 | isbn_softcover | 978-1-4939-6210-5 | isbn_ebook | 978-1-61779-536-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2012 |
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