Titlebook: Neurotransmitter Transporters; Investigative Method Heinz Bönisch,Harald H. Sitte Book 2016 Springer Science+Business Media New York 2016 n

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Book 2016t ensures successful results in the laboratory..Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in thefurther study of this vital field..

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Animal Models for Depression and the Mode of Action of Neurotransmitter Transporter-Blocking Antident effects on depression-like behavior. Here we present the . model as a suitable paradigm for eliciting depression-like behavior in mice. We describe the procedures used to interrogate the model by quantifying the induced behavioral phenotype, the associated physiological alterations and their modulation by drug candidates.

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Organic Cation Transport Measurements Using Fluorescence Techniques,zed in a wide spectrum of experimental settings analyzing such transport dynamics from the in vitro to the in vivo situation. Since its first introduction in 1994, ASP. has been widely used by several laboratories worldwide for all kinds of studies analyzing organic cation transport.

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Tracer Flux Measurements to Study Outward Transport by Monoamine Neurotransmitter Transporters,s still enigmatic and involves complex regulatory processes. In the current chapter, we describe various methods that can be used to assess the efflux of neurotransmitter from cells heterologously expressing the NTTs of interest or from preparations derived from intact brain tissue.

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Classical Radioligand Uptake and Binding Methods in Transporter Research: An Emphasis on the Monoamt since the late 1950s, and they continue to contribute major insights into transporter function and structure to date. In the current chapter, we focus primarily on the family of monoamine (MA) neurotransmitter transporters (MATs), i.e., transporters (T) for norepinephrine [noradrenaline] (NET), do

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Tracer Flux Measurements to Study Outward Transport by Monoamine Neurotransmitter Transporters,plish uptake by undergoing a transport cycle, which relies on a return step in the empty state. In addition, NTTs can also run in the reverse direction and transport substrates out of the cells. This can be observed under conditions, where the transmembrane sodium gradient dissipates, e.g., if sodiu

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