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Titlebook: Neuroproteomics; Ka Wan Li Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2019 rodent brain regions

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发表于 2025-3-21 16:38:58 | 显示全部楼层 |阅读模式
书目名称Neuroproteomics
编辑Ka Wan Li
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Neuromethods
图书封面Titlebook: Neuroproteomics;  Ka Wan Li Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2019 rodent brain regions
描述This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In .Neuromethods. series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory..Cutting-edge and comprehensive, .Neuroproteomics, Second Edition. is a valuable guide that help researchers obtain the finest results from current proteomics platforms..
出版日期Book 2019Latest edition
关键词rodent brain regions; synaptic organelles; Laser capture microdissection; pluripotent brain stem cells;
版次2
doihttps://doi.org/10.1007/978-1-4939-9662-9
isbn_softcover978-1-4939-9664-3
isbn_ebook978-1-4939-9662-9Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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发表于 2025-3-22 00:11:47 | 显示全部楼层
Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip,0 min per sample. This micropipette-tip method would be thus used preferentially as an alternative to existing tools for the reliable enrichment of phosphorylated biomolecules, such as phosphopeptides, in the field of neuroscience.
发表于 2025-3-22 03:15:46 | 显示全部楼层
,Integrated Immunoprecipitation: Blue Native Gel Electrophoresis—Mass Spectrometry for the Identificlexes. Here, we describe a protocol that combines the specificity of immunoprecipitation, the size separating capacity of Blue native polyacrylamide gel electrophoresis, and subsequent mass spectrometry to delineate the protein constituents in subcomplexes of a targeted protein.
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High-pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics,tide mixtures, in which the HPLC-based reversed-phase fractionation provides higher resolving power while the tip-based high-pH reversed-phase fractionation is simple, parallelizable, and suitable for limited amount of biological samples.
发表于 2025-3-22 19:37:59 | 显示全部楼层
,Analysis of Synaptic Protein–Protein Interaction by Cross-linking Mass Spectrometry, hybrid analysis, this protocol could characterize protein–protein interactions in a more physiological setting and allows the detection of native protein complexes contained in the mouse synaptosome.
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2D-DIGE as a Tool in Neuroproteomics,esis (2D-DIGE) currently being the most comprehensive technique to quantify these changes while retaining information about isoforms and posttranslational modifications. This chapter focusses on technical aspects of 2D-DIGE as well as a brief overview of successful applications of this technique in neuroproteomics.
发表于 2025-3-23 06:44:50 | 显示全部楼层
A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder,ctional pathways and interactome connections of selected risk genes. The overlay of pathway and proteome data from selected genes in a biological context can be used to formulate new testable hypothesis of both the genetics and the biology of the disorders.
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