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Titlebook: Neuronal Cell Death; Methods and Protocol Laura Lossi,Adalberto Merighi Book 2015 Springer Science+Business Media New York 2015 DNA synthes

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Nuclear Signs of Pre-neurodegenerationrange of neurodegenerative disorders. The tools here described may be of use for the early detection of pre-degenerative processes in neurodegenerative diseases and for validating novel rescue strategies.
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Calcium Imaging in Neuron Cell Deathpalratneurons and fluorescence imaging of cytosolic Ca2+concentration in Fura2/AM-loaded neurons are described. We also describe a method for apoptosis detection by immunofluorescenceimaging. Finally, a simple method for concurrent measurements of [Ca2+]cyt and apoptosis in the same neurons is described.
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Image Analysis Algorithms for Immunohistochemical Assessment of Cell Deathle more objective and accurate morphometricquantification of cell injury in whole brain sections. In this chapter, the proposed workflow describes what tasks need to be fulfilled to visualize and gauge cell death characterization by histologicalstains and immunohistochemicalmarkers.
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