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Titlebook: Natural Killer Cell Protocols; Cellular and Molecul Kerry S. Campbell,Marco Colonna Book 20001st edition Humana Press 2000

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Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/661776.jpg
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Natural Killer Cell Protocols978-1-59259-044-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Cloning Human Natural Killer Cells,is has prompted the production of monoclonal antibodies directed to NK cell surface antigens clonally distributed, and, ultimately, the biochemical and molecular definition of the NK cell surface glycoproteins functioning as MHC class I receptors.
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Generation of HLA Class I Transfected Target Cell Lines,t has proved useful to use transfected target cells that express only one class I allele, so that the effects of individual class I allotypes can be compared directly using the same target cell background.
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Assays for Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Reverse ADCC (Redirected Cytotoand therefore target cells that, not recognized spontaneously by NK cells, are sensitized with anti-target cell antibodies (Abs) of the IgG class. The two types of cytotoxicity elicited are referred to as “spontaneous” and “Ab-dependent’ cell-mediated cytotoxicity (ADCC).
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