书目名称 | Natural Killer Cell Protocols | 副标题 | Cellular and Molecul | 编辑 | Kerry S. Campbell,Marco Colonna | 视频video | http://file.papertrans.cn/662/661776/661776.mp4 | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I trans | 出版日期 | Book 20001st edition | 版次 | 1 | doi | https://doi.org/10.1385/1592590446 | isbn_ebook | 978-1-59259-044-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2000 |
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