Titlebook: Natural Killer Cell Protocols; Cellular and Molecul Kerry S. Campbell Book 2010Latest edition Humana Press 2010 Activation.CMV.Malaria.PCR.

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Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NKcells, the establishment of a well-defined protocol to replicate in vitro the major steps corresponding to the process of NK cell differentiation and maturation has enabled us to dissect the molecular events governing certain aspects of NK cell development. This chapter describes a protocol that com

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Use of Transfected , S2 Cells to Study NK Cell Activation,ory NK cell receptors. Mammalian target cells typically express a variety of ligands for NK cell receptors. Engagement of NK cell receptors by antibodies may not mimic activation by natural ligands. To define requirements for activation and dissect the contribution of receptors to NK cell function,

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Analysis of the NK Cell Immunological Synapse, NK cell and the cell with which it is interacting. This interface is also known as the immunological synapse and is characterized by an extraordinary number of dynamic molecular events that have the potential to result in NK cell function. Here we describe microscopy-based methods for evaluating an

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Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry,ometer fitted with a UV laser. One major advantage of this method is that the calcium response of the minority NK cell population and even smaller NK cell subpopulations can be measured simultaneously from a mixture of freshly prepared total splenocytes without resorting to prior cell sorting or exp

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Lentiviral Gene Transduction in Human and Mouse NK Cell Lines,ce of these cells to gene transfer. Considering over-expression and gene knockdown studies are crucial tools to study the biology of cells, technologies suitable for transfering genes into NK cells are invaluable. Among various technologies available for gene transfer, lentiviral-mediated transducti