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Titlebook: Natural Killer Cell Protocols; Cellular and Molecul Kerry S. Campbell Book 2010Latest edition Humana Press 2010 Activation.CMV.Malaria.PCR.

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书目名称Natural Killer Cell Protocols
副标题Cellular and Molecul
编辑Kerry S. Campbell
视频video
概述Remains the most comprehensive collection of methods available for studying natural killer cells in both mice and humans.Examines a wide array of molecular and cellular techniques for manipulating and
丛书名称Methods in Molecular Biology
图书封面Titlebook: Natural Killer Cell Protocols; Cellular and Molecul Kerry S. Campbell Book 2010Latest edition Humana Press 2010 Activation.CMV.Malaria.PCR.
描述.As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of "Natural Killer Cell Protocols: Cellular and Molecular Methods" brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume
出版日期Book 2010Latest edition
关键词Activation; CMV; Malaria; PCR; Promoter; Transplantation; Vivo; cell lines; genes; inbred mouse strains; infec
版次2
doihttps://doi.org/10.1007/978-1-60761-362-6
isbn_softcover978-1-4939-5624-1
isbn_ebook978-1-60761-362-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2010
The information of publication is updating

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Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction,f EGFP or the transduced cell surface marker. Alternatively, cells expressing the transduced cDNAs can be selected for by treatment with neomycin, puromycin, or hygromycin. Using this method, the sorted/selected cells uniformly express the gene of interest and the expression is stable for many weeks of culture.
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Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils,elopment, as they exist in vivo, through analysis of NK cell maturational intermediates found in situ. Herein we describe a detailed, yet simple, four-step protocol for the viable enrichment and purification of human NK cell developmental intermediates from LNs and tonsils.
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Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry,equently cross-linked by addition of streptavidin. The increase in intracellular calcium is quantified by measuring a shift in the indo-1 emission spectrum that takes place when the dye becomes bound to calcium.
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