Titlebook: Nanopore Technology; Methods and Protocol Monifa A.V. Fahie Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Pr

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Preparation of Fragaceatoxin C (FraC) Nanopores via two rounds of Ni-NTA affinity chromatography before and after oligomerization on sphingomyelin-containing liposomes. Starting from a plasmid vector containing the FraC gene, our method allows the production of purified nanopores within a week. Afterward, the FraC nanopores can be stored at +4 °C for several months, or frozen.

ANIM

Nanopore Enzymology to Study Protein Kinases and Their Inhibition by Small Moleculesnd production of suitably engineered protein nanopores and their use for the determination of key mechanistic parameters of kinases. We also provide procedures for the determination of inhibition constants of protein kinase inhibitors.

ALIAS

水槽

Oligonucleotide-Directed Protein Threading Through a Rigid Nanoporepore inserted in an electrically insulating membrane. The translocation of the protein molecule through the pore causes a modulation of the ionic current. Analysis of the ionic current reveals the biophysics of co-translocational unfolding and may be used to infer the amino acid sequence and posttranslational modifications of the molecule.

较早

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Design and Assembly of Membrane-Spanning DNA NanoporesVersatile lipid membrane-inserting nanopores have been made by functionalizing DNA nanostructures with hydrophobic tags. Here, we outline design and considerations to obtain DNA nanopores with the desired dimensions and conductance properties. We further provide guidance on their reconstitution into lipid membranes.

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Nanopore Technology978-1-0716-0806-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

敲竹杠

Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/660911.jpg

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