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Titlebook: Nanopore Technology; Methods and Protocol Monifa A.V. Fahie Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Pr

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发表于 2025-3-21 17:32:23 | 显示全部楼层 |阅读模式
书目名称Nanopore Technology
副标题Methods and Protocol
编辑Monifa A.V. Fahie
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Nanopore Technology; Methods and Protocol Monifa A.V. Fahie Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Pr
描述This detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Nanopore Technology: Methods and Protocols., with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are
出版日期Book 2021
关键词Protein analytes; Single molecule detection; DNA sequencing; Membrane channels; Nanopore sensing; Translo
版次1
doihttps://doi.org/10.1007/978-1-0716-0806-7
isbn_softcover978-1-0716-0808-1
isbn_ebook978-1-0716-0806-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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Free Energy Minimization for Vesicle Translocation Through a Narrow Porehe free energy landscape of the vesicle in terms of bending and stretching energy and use Fokker-Plank formalism to calculate the first-passage translocation time. We also address various modifications that can be done to this approach to make it work for different systems.
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Book 2021tions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, ch
发表于 2025-3-22 10:48:05 | 显示全部楼层
1064-3745 expertsThis detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to
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Preparation of Cytolysin A (ClyA) Nanoporesre, we describe a simple protocol allowing the production of ClyA nanopores. Monomers of ClyA are expressed in . and oligomerized in the presence of detergent. Subsequently, different oligomer variants are electrophoretically resolved and stored in a gel matrix for long-term use.
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Determining the Orientation of Porins in Planar Lipid Bilayershod for determining the orientation of porins in a PLB—with a focus on . OmpF—by using targeted covalent modification of cysteine mutants. Each of the two possible orientations can be correlated with the porin conductance asymmetry, such that thereafter an .–. curve taken at the start of an experiment will suffice to establish orientation.
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