Titlebook: NLR Proteins; Methods and Protocol Pablo Pelegrín,Francesco Di Virgilio Book 2023Latest edition The Editor(s) (if applicable) and The Autho

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Petra Sušjan-Leite,Iva Hafner-Bratkovič These constituents exert forces on one another which decrease with the distance between them. When thermal agitation, which increases with the temperature of the system, is stronger than the interaction forces which tend on the whole to bring the constituent elements together, the matter is in the

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Arinna Bertoni,Ignazia Prigione,Sabrina Chiesa,Isabella Ceccherini,Marco Gattorno,Anna Rubartellihe same blood group as the patient are used, transfusions are practically free from danger. Even after this discovery was generally adopted and applied, however, occasional hemolytic reactions continued to occur. In the earlier days, practically all of these reactions could be traced to mistakes in

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1064-3745 ation advice from the experts.This second edition provides a sound basis for the molecular investigation of NLR function in health and disease. Chapters focus on NLR as innate immune receptors, “atypical” inflammasomes, biochemical and novel bioluminescence techniques for the measurement of IL-1b as

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Book 2023Latest editionune receptors, “atypical” inflammasomes, biochemical and novel bioluminescence techniques for the measurement of IL-1b as readout of NLR-based inflammasomes, bioluminescent probe, biochemical and microscopy techniques, techniques to measure caspase-1 activation, cell free systems for the study of in

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Measuring NLR Oligomerization III: Detection of NLRP3 and NLRC4 Complex by Bioluminescence Resonanc could be used to study protein-protein interactions and conformational changes within proteins at real-time in living cells. This protocol describes the use of BRET technique to study NLRP3 oligomerization in living cells before and during NLRP3 inflammasome activation.