Titlebook: NLR Proteins; Methods and Protocol Pablo Pelegrín,Francesco Di Virgilio Book 2023Latest edition The Editor(s) (if applicable) and The Autho

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Assessing the ATP Binding Ability of NLRP3 from Cell Lysates by a Pull-down Assay,ing is followed after elution from the beads and detection with Western blot and immunolabelling. The method can be used to evaluate the functionality of NLRP3 variants or to check whether compounds or NLRP3 binding partners interfere with binding of ATP.

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Inflammasome-Independent Roles of NLR and ALR Family Members,ers exert inflammasome-independent functions. Here, we will discuss inflammasome-independent functions of NLRC4, NLRP12, and AIM2 and examine their roles in regulating innate and adaptive immune processes.

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Theoretical 3D Modeling of NLRP3 Inflammasome Complex,n structural modeling that provides unique insights into the biological role and activation of NLRP3 oligomer. Finally, the same approach can be easily adapted to the rest of the members of the NLRP family.

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Methods to Measure NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, an the oligomeric state with high sensitivity and stability. ASC oligomerization has been successfully used as readout for NLR/ALR inflammasome activation in response to various PAMPs and DAMPs in human and mouse macrophages and THP-1 cells. Here, we provide a detailed description of the methods used