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Titlebook: Microinjection and Transgenesis; Strategies and Proto Angel Cid-Arregui,Alejandro García-Carrancá Book 1998 Springer-Verlag Berlin Heidelbe

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Carol Murphy,Luis Martin-Parras,Ulrich Rüthernominal and worst-case operating conditions in modern microprocessor designs grows, the inefficiencies of worst-case design are too large to ignore. Recognizing the inefficiencies in such a design style, researchers have begun to propose architecture-level solutions that address worst-case condition
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tion of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods978-3-540-61895-9978-3-642-80343-7
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Preparation, Purification, and Analysis of Synthetic Oligonucleotides Suitable for Microinjection Exesired amount of oligonucleotides inside the cells. Oligonucleotides are injected into cells to inhibit gene expression (Kleuss et al. 1994; Kola and Sumarsono 1995; Moulds et al. 1995) and to analyze the fate of antisense oligonucleotides in cells (Chin et al. 1990; Dagle et al. 1991; Leonetti et al. 1991).
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Manual Microinjection: Measuring the Inhibitory Effect of HIV-1-Directed Hammerhead Ribozymes in Tis-1 replication was quantified by measurement of the release of infectious HIV-1 particles after amplification of HIV-1 produced in initially microinjected SW480 cells by cocultivated CD4. MT-4 cells (Sczakiel et al. 1990).
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Videomicroscopy of Living Microinjected Hippocampal Neurons in Cultureris et al. 1988), and actin dynamics in growth cones (Forscher and Smith 1988). Beside these methods, organelle transport can be also specifically visualized and documented by fluorescent microscopy using specific fluorescent probes (Lee and Chen 1988).
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