Titlebook: Microinjection and Transgenesis; Strategies and Proto Angel Cid-Arregui,Alejandro García-Carrancá Book 1998 Springer-Verlag Berlin Heidelbe

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ation and full practical details (materials needed, exact reThe establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ­ enced many fields of biology

不规则的跳动

Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysular recording were developed in the late 1940s. Less than one decade later, similar techniques were applied to perform direct transfer of biological material into cells (see Chambers and Chambers 1961). Initially, microinjection was designed for transplantation of mammalian nuclei (Graessmann 1970)

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Automated Computer-Assisted Microinjection into cultured somatic cellsn (for an overview see Celis 1986), and is now established as one of the most versatile methods of introducing substances into such living cells. Microinjection made it possible to use single cells as objects to study complex cellular processes, structure and function in vivo. A large variety of mol

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Videomicroscopy of Living Microinjected Hippocampal Neurons in Culture types of videomicroscopy will be described: videomicroscopy with normal phase contrast, and video enhanced contrast differential interference contrast (VECDIC) microscopy. The first is especially suited to study morphological changes of the cell such as cell movement, retraction, outgrowth, filopod

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Cellular Microbiochemistryfer can occur at well-defined stages of the cell cycle, and modifications of culture conditions are possible before, during, and after injection. The number of cells which can be injected per experiment is, however, limited. Therefore, in the past, biochemical analyses of microinjected cells has bee