Titlebook: Microinjection and Transgenesis; Strategies and Proto Angel Cid-Arregui,Alejandro García-Carrancá Book 1998 Springer-Verlag Berlin Heidelbe

不理会

Immunofluorescence and Immunoelectron Microscopy of Microinjected and Transfected Cultured Cellsion of a significant number of molecules (Melan and Sluder 1992; Griffiths et al. 1993). Therefore, if the precise localization of a protein is being studied, it will always be necessary to look at the fine structure of the cell, using immunoelectron microscopy techniques. Nevertheless, whenever pos

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Tremor

Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis?bably been a rare event. One experimental model is provided by a strain of laboratory mice (SWR/J), where efficient provirus acquisition in the female germline has been reported (Jenkins et al. 1985; Panthier et al. 1988).

逃避责任

The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stem the mutated allele (see for review Capecchi 1989). ES cell technology has also allowed the use of gene trap strategies to isolate and mutate genes relevant in mouse development (Gossler et al. 1989). Methods for derivation, culture, and genetic modification of ES cells, either by gene targeting or

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The Generation of Transgenic Mice with Yeast Artificial Chromosomescontaining all the necessary elements for stable maintenance of the artificial chromosome in yeast cells. Telomeric sequences guarantee chromosomal stability. The long vector arm harbors a centromer and an autonomously replicating sequence required for chromosomal segregation and replication, respec

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ERUPT

Mhc-Molecule

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Gustavo Egea,Teresa Babia,Roser Pagan,Roser Busca,Inmaculada Ayala,Ferran Valderrama,Manuel Reina,Seironment. Thus, monitoring and analysis of these compounds become critical; however, it is very challenging and requires tedious sample preparation and sophisticated analytical instrumentation, such as gas/liquid chromatography coupled with mass spectroscopy (GC/LC-MS). The current chapter focuses o

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