Titlebook: Microinjection; Methods and Applicat David J. Carroll Book 2009 Humana Press 2009 Antibodies.Expression.Gene function.Nucleotide.Protein ex

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Laurinda A. Jaffe,Rachael P. Norris,Marina Freudzon,William J. Ratzan,Lisa M. Mehlmannca. The communities of Cape Town responded in a timely way to the risks associated with the pandemic to limit its dangerous potential and find solutions to support people in critical situations. These are due to existing critical medical conditions (TB and HIV rates), impossibility to practice physi

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Vincenzo Cavalieri,Maria Di Bernardo,Giovanni Spinelliainable cities by development agencies, governments, and academics. Urbanisation brings about challenges in various aspects of living, from transportation and mobility to housing. Factors like uneven economic development, mass consumerism, and excessive utilisation of natural resources, which are co

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Satoshi Kishigami,Teruhiko Wakayama public safety, transportation and poor water supply. Small businesses can take advantage of these opportunities to promote Smart Communities in cities. Although Smart City and Smart Community initiatives in South Africa are in their infancy, they can complement existing Integrated Development Plans

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,Use of Luciferase Chimaera to Monitor PLCζ Expression in Mouse Eggs,ein that is expressed in eggs after injection of cRNA is effective in causing Ca. oscillations at very low concentrations. In order to measure the amount and timecourse of protein expression we have tagged PLCζ with firefly luciferase. The expression of the luciferase protein tag in eggs is then mea

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A Microinjectable Biological System, the , Oocyte, as an Approach to Understanding Signal Transductertebrate cell, the . oocyte, is a perfect model system to perform these studies. Oocytes are numerous and synchronous cells, arrested in the G2 phase of the cell cycle and easily amenable to biochemical, electrophysiological, and cytological studies. We describe how to microinject proteins or pepti

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