| 书目名称 | Microinjection | | 副标题 | Methods and Applicat | | 编辑 | David J. Carroll | | 视频video | | | 概述 | Explores a wide variety of model systems, including human, mouse, sea urchin, starfish, zebrafish, and Xenopus oocytes, transformed Caenorhabditis elegans, and tissue culture cells.Contains detailed m | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In .Microinjection: Methods and Applications., expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in .Xenopus. oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful .Methods in Molecular Biology.™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls | | 出版日期 | Book 2009 | | 关键词 | Antibodies; Expression; Gene function; Nucleotide; Protein expression; RNA expression; Regulation; gene exp | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-59745-202-1 | | isbn_softcover | 978-1-4939-6090-3 | | isbn_ebook | 978-1-59745-202-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2009 |
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发表于 2025-3-21 18:40:12
