书目名称 | Microfluidics for Single-Cell Analysis | 编辑 | Jin-Ming Lin | 视频video | | 概述 | Summarizes the various microfluidic-based single cell analysis methods.Reviews the state-of-the-art techniques showcasing the pros and cons of each method in details.Offers lessons learned and tips fr | 丛书名称 | Integrated Analytical Systems | 图书封面 |  | 描述 | .This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementa | 出版日期 | Book 2019 | 关键词 | Single Cell Analysis; Cell Co-culture; Mass Spectrometry; Microfluidics; Cell Isolation and Recognition; | 版次 | 1 | doi | https://doi.org/10.1007/978-981-32-9729-6 | isbn_softcover | 978-981-32-9731-9 | isbn_ebook | 978-981-32-9729-6Series ISSN 2196-4475 Series E-ISSN 2196-4483 | issn_series | 2196-4475 | copyright | Springer Nature Singapore Pte Ltd. 2019 |
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