Titlebook: Microfluidic Techniques; Reviews and Protocol Shelley D. Minteer Book 2006 Humana Press 2006 DNA.PCR.cellular processes.ethanol.migration

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Rabih Zaouk,Benjamin Y. Park,Marc J. Madouof treatment alterna­ tives and to empirically determine the effectiveness of specific interventions is particularly great. Residential and inpatient treatment is also an expensive and limited resource, and our wise utilization of it should be guided by a comprehensive understanding of its benefits

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Rabih Zaouk,Benjamin Y. Park,Marc J. Madouire range of treatment alterna­ tives and to empirically determine the effectiveness of specific interventions is particularly great. Residential and inpatient treatment is also an expensive and limited resource, and our wise utilization of it should be guided by a comprehensive understanding of its benefits 978-1-4899-0929-9978-1-4899-0927-5

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Laser Ablation as a Fabrication Technique for Microfluidic Devices such a technique, and this chapter describes the fabrication of a simple injection tee utilizing this technique. This chapter also examines the practical details involved in laser ablation from the perspective of a scientist who desires to utilize a turn-key system.

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A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes to perform a typical sandwich assay. By using a high-resolution fluorescent microscope, individual nanoparticle- DNA probes that have been hybridized to capture target strands can be observed clearly at low target DNA concentrations. More important, the number of the nanoparticle-DNA probes hybridi

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Microscale Integrated Sperm Sorterding and sorting functions necessary to prepare high-quality sperm for in vitro fertilization. This self-contained, inexpensive, and portable device may also be useful for developing convenient sperm motility assays that can be used at the point of care or at home.

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Reversible, Room Temperature Bonding of Glass Devices for Microfluidicsrd particles, cells, or other biological material, reversible bonding provides a way of increasing the chip’s lifetime and utility. Glass microdevices that are cleaned following this procedure can be successfully used many times for electrophoresis or pressure-driven applications.

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Polymerase Chain Reaction in Miniaturized Systems:ess these needs, combining the ability to perform rapid thermocycling with a portable device format that can be inexpensively mass produced. We review recent efforts aimed at developing these next-generation systems and discuss some of the practical considerations involved in adapting this technology for routine laboratory use.