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Titlebook: Microfluidic Techniques; Reviews and Protocol Shelley D. Minteer Book 2006 Humana Press 2006 DNA.PCR.cellular processes.ethanol.migration

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书目名称Microfluidic Techniques
副标题Reviews and Protocol
编辑Shelley D. Minteer
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Microfluidic Techniques; Reviews and Protocol Shelley D. Minteer Book 2006 Humana Press 2006 DNA.PCR.cellular processes.ethanol.migration
描述Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
出版日期Book 2006
关键词DNA; PCR; cellular processes; ethanol; migration
版次1
doihttps://doi.org/10.1385/1592599974
isbn_softcover978-1-61737-613-9
isbn_ebook978-1-59259-997-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2006
The information of publication is updating

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Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Moldsther techniques such as injection molding are more apt for mass manufacturing and cost-effective, PDMS molding is used almost exclusively for rapid prototyping in corporate and research environments because of its simplicity and fast turnaround time.
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Solid-Phase Extraction in Packed Beds on Glass Microdevicess are included for the packing and use of the bead bed for solid-phase extraction (SPE). The SPE procedure may be used, e.g., as a filter to clean up a dirty sample prior to analysis, or as a means of pre-concentration for a dilute sample. Once the bead bed is in place, it may be used multiple times without sample breakthrough.
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Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/633257.jpg
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https://doi.org/10.1385/1592599974DNA; PCR; cellular processes; ethanol; migration
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Gloria Thomas,Emad M. El-Giar,Laurie E. Locascio,Michael J. Tarlov.
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Shelley D. Minteer,Christine M. Mooreire range of treatment alterna­ tives and to empirically determine the effectiveness of specific interventions is particularly great. Residential and inpatient treatment is also an expensive and limited resource, and our wise utilization of it should be guided by a comprehensive understanding of its benefits 978-1-4899-0929-9978-1-4899-0927-5
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