书目名称 | Microfluidic Techniques | 副标题 | Reviews and Protocol | 编辑 | Shelley D. Minteer | 视频video | | 概述 | Includes supplementary material: | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. | 出版日期 | Book 2006 | 关键词 | DNA; PCR; cellular processes; ethanol; migration | 版次 | 1 | doi | https://doi.org/10.1385/1592599974 | isbn_softcover | 978-1-61737-613-9 | isbn_ebook | 978-1-59259-997-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2006 |
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