Titlebook: Microchip Diagnostics; Methods and Protocol Valérie Taly,Jean-Louis Viovy,Stéphanie Descroix Book 2017 Springer Science+Business Media LLC

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Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reactionil how to build up an electrowetting-on-dielectric-based digital microfluidic chip with unique advantages for performing single-molecule detection. We illustrate how superparamagnetic particles can be printed with very high loading efficiency (over 98 %) and single-particle resolution in the microwe

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Microfluidic DNA Stretching Device for Single-Molecule Diagnosticsodification of the DNA or surface modification of the substrate. To detect a signal variation caused by sequence-specific dye binding or partial melting, it is crucial that the DNA molecules are arrayed in a parallel direction inside the narrow microscopic field. The method uses zigzag-shaped microg

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SlipChip Device for Digital Nucleic Acid Amplificationpartitions and performing parallel nucleic acid amplification, such as polymerase chain reaction (PCR) or isothermal amplification reactions. With the counts of positive wells, total number of wells, and volumes of wells, the concentration of the target nucleic acid in the sample can be quantified.

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Multiplex Detection of , Mutations Using Passive Droplet Fusionnomic DNA from H1573 cell-line was screened for the presence of the six common mutations of the . oncogene as well as wild-type sequences with a detection efficiency of 98 %. Furthermore, the mutant allelic fraction of the cell-line was also assessed correctly showing that the technique is quantitat

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Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chipspecifically as a companion diagnostic for new anticancer drugs, and for research into the mechanisms of disease progression and metastases. Ideally, CTCs would be enriched from very small samples, with minimal handling, high recovery, and no requirement for the expression of specific surface marker

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Microfluidic-Based Bacteria Isolation from Whole Blood for Diagnostics of Blood Stream Infection shock, and multi organ failure syndrome. Blood culturing is currently the gold standard for the identification of microorganisms and, although it has been automated over the decade, the process still requires 24–72 h to complete. This long turnaround time, especially for the identification of antim

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