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Titlebook: Microchip Diagnostics; Methods and Protocol Valérie Taly,Jean-Louis Viovy,Stéphanie Descroix Book 2017 Springer Science+Business Media LLC

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发表于 2025-3-21 18:25:29 | 显示全部楼层 |阅读模式
书目名称Microchip Diagnostics
副标题Methods and Protocol
编辑Valérie Taly,Jean-Louis Viovy,Stéphanie Descroix
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Microchip Diagnostics; Methods and Protocol Valérie Taly,Jean-Louis Viovy,Stéphanie Descroix Book 2017 Springer Science+Business Media LLC
描述.This volume illustrates how microfluidic approaches can meet the requirement of clinical diagnosis based on molecular or cellular biomarkers. .Microchip Diagnostics: Methods and Protocols is .divided into four sections describing the business aspect.s. of the microfluidic-based systems for diagnosis, demonstrating how versatile microfluidics can be regarding to protein bioassay integration, presenting microfluidic approaches for nucleic analysis based on mono or diphasic format, and highlighting recent contributions. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..
出版日期Book 2017
关键词microfluidic approaches; molecular biomarkers; cellular biomarkers; cell analysis; microfluidics; protei
版次1
doihttps://doi.org/10.1007/978-1-4939-6734-6
isbn_softcover978-1-4939-8286-8
isbn_ebook978-1-4939-6734-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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发表于 2025-3-22 00:03:31 | 显示全部楼层
Using Electrophoretic Immunoassay to Monitor Hormone Secretiontrophoresis assay that allows cell culture and hormone monitoring. An online gradient generator can control cell culture condition precisely. This system has been applied for Pancreas islets’ glucose sensitivity studies.
发表于 2025-3-22 03:03:27 | 显示全部楼层
On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnosticsnd methodology for performing rapid immunoassays on magnetic particles in continuous flow via their deflection through multiple laminar flow streams of reagents and washing solutions. In particular, we focus on the use of the microfluidic platform for a C-reactive protein (CRP) sandwich immunoassay in less than 60 s.
发表于 2025-3-22 04:39:24 | 显示全部楼层
Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic ChipCTCs would be enriched from very small samples, with minimal handling, high recovery, and no requirement for the expression of specific surface markers. Here, we describe negative enrichment as the preferred approach for cancer cell isolation using a microfluidic platform.
发表于 2025-3-22 10:32:10 | 显示全部楼层
Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reactionll array patterned in the Teflon-AF. surface of the grounding plate of the chip. Finally, the potential of the device for its application to single-molecule detection is demonstrated by the ultrasensitive detection of the biotinylated enzyme β-Galactosidase captured on streptavidin-coated particles in the described platform.
发表于 2025-3-22 13:59:49 | 显示全部楼层
Microfluidic DNA Stretching Device for Single-Molecule Diagnosticsrooves in a densely-arranged molecular patterning apparatus in a microfluidic channel. By syringing through the microchannel, over 1500 DNA molecules can be arrayed simultaneously in the microgrooves. It will therefore serve as a template preparation for DNA molecular diagnosis by high-resolution imaging.
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发表于 2025-3-23 02:17:53 | 显示全部楼层
Droplet Microfluidic and Magnetic Particles Platform for Cancer Typingown to the single cell level..We report on a droplet microfluidic platform based on the manipulation of magnetic particles that allows the clinical analysis of tumor tissues. In particular, it allows the extraction of mRNA from the total-RNA sample, Reverse Transcription, and cDNA amplification, all in droplets.
发表于 2025-3-23 08:53:38 | 显示全部楼层
Book 2017t, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..
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