Titlebook: Microbial Toxins; Methods and Protocol Otto Holst Book 2017Latest edition Springer Science+Business Media LLC 2017 Clostridium tetani.aflat

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Otto HolstIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia

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1064-3745 ation advice from the experts.Includes supplementary materia.This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in .Microbial Toxins: Met

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Aptamer-Based Trapping: Enrichment of , Spores for Real-Time PCR Detection,ween 0.3 and 3.5%. Thereby, enrichment factors of up to sixfold were achieved. The combination of an aptamer-based enrichment by magnetic separation and the subsequent specific real-time PCR detection represents a reliable and rapid detection system.

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Detection of , in Complex Matrices by Intact Cell Immunocapture and Targeted Mass Spectrometry, toxine, plasminogen activator and pesticin) are monitored by targeted LC-MS/MS using the selected reaction monitoring (SRM) mode. This immuno-LC–MS/MS assay has a limit of detection of 2 × 10. CFU/mL in milk or tap water, and 4.5 × 10. CFU in 10 mg of soil.

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Endotoxin Removal from , Bacterial Lysate Using a Biphasic Liquid System, water immiscible solvent such as 1-octanol. During extraction most of the phage lytic activity is retained in the aqueous phase, while endotoxin accumulates in the organic solvent. The levels of endotoxin in the aqueous bacteriophage rich fraction are determine by . or EndoLISA assay and are extremely low.

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Molecular Methods for Identification of , by Targeting Neurotoxin,producing (.) gene-targeted methods using .-specific primers. Here, we describe various molecular methods targeting . gene such as polymerase chain reaction, pulsed-field gel electrophoresis, Southern blotting, loop-mediated isothermal amplification assay, etc. to confirm the virulence of ..