Titlebook: Microbial Steroids; Methods and Protocol Carlos Barreiro,José-Luis Barredo Book 2023Latest edition The Editor(s) (if applicable) and The Au

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Identification and Characterization of Some Genes, Enzymes, and Metabolic Intermediates Belonging toeria able to assimilate bile acids and/or other steroids (e.g., testosterone and 4-androsten-3,17-dione) under aerobic conditions through the 9,10-. pathway. From soil samples, we have isolated several strains belonging to genus . that grow efficiently in chemically defined media containing some cyc

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RNA Preparation and RNA-Seq Bioinformatics for Comparative Transcriptomicsion sequencing of RNA (RNA-seq) has several advantages over other techniques, such as the capability of detecting all the transcripts in one assay over RT-qPCR, such as its higher accuracy and broader dynamic range over microarrays or the ability to detect novel transcripts, including non-coding RNA

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Bidimensional Analyses of the Intra- and Extracellular Proteomes of Steroid Producer Mycobacteria industrially relevant mycobacteria have received less attention, even though the steroid market sales in 2021 were estimated in $56.45 billion..The extracellular proteome, due to its relevance in the sterol processing and uptake, and the intracellular proteome, because of its role in steroids bioco

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β-Sitosterol Bioconversion in Small-Scale Devices: From Microtiter Plates to Microfluidic Reactorsy under similar conditions and replicate all functions of bench-scale reactors at dramatically smaller volumes. Microtiter plates, due to the standard footprint, can be integrated with liquid handling systems and associated equipment, expanding considerably their application and use. However, care h