Titlebook: Microbial Proteomics; Methods and Protocol Dörte Becher Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Subcel

Projection

Mnemonics

Applications of Difference Gel Electrophoresis (DIGE) in the Study of Microorganismsce changes on a quantitative and statistically confident level—in particular also for laboratories not having full-cycle proteomic facilities at their disposal. In this contribution we summarize our methodological procedures and experiences with 2D DIGE accumulated over the past 15 years.

pellagra

SPURN

1064-3745 sults.Contains key notes and implementation advice from the This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcel

甜得发腻

Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobi peripheral membrane proteins), and the other part targeting primarily protein domains spanning the lipid bilayer. The feasibility of the protocol, as it is described here, was originally shown for the gram-positive pathogenic bacterium . but should be applicable to any kind of membrane protein.

attenuate

How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in of a single protein is radioactive pulse-chase labeling combined with immunoprecipitation. Besides a detailed description of the standard protocol, the general applicability as well as certain improvements of the method will be discussed here.

conception

innate

织布机

Protein Enrichment from Highly Dilute Samples with StrataClean fluids. Traditionally several precipitation strategies were used for this purpose, but they have certain drawbacks. Here, a protocol for StrataClean enrichment—a very efficient and easy-to-use method for the enrichment of proteins from highly dilute samples—which is compatible to gel-free proteomic

foppish

Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobition rates with respect to the challenging class of membrane proteins. This will increase comprehensiveness of global proteome studies on the one hand but could also be of interest for researchers targeting specific membrane proteins or membrane protein sequences on the other hand. The protocol is a