Titlebook: Microbial Proteomics; Methods and Protocol Dörte Becher Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Subcel

恰当

书目名称Microbial Proteomics影响因子(影响力)




书目名称Microbial Proteomics影响因子(影响力)学科排名




书目名称Microbial Proteomics网络公开度




书目名称Microbial Proteomics网络公开度学科排名




书目名称Microbial Proteomics被引频次




书目名称Microbial Proteomics被引频次学科排名




书目名称Microbial Proteomics年度引用




书目名称Microbial Proteomics年度引用学科排名




书目名称Microbial Proteomics读者反馈




书目名称Microbial Proteomics读者反馈学科排名

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眼界

Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)les. Once set up, the workflow relies on defined chemical or commercially available complex media leading to universal heavy labeling by the stable isotope .N. This chapter provides a detailed protocol for labeling of different bacteria and the strategy for evaluating the incorporation rate of the heavy stable isotopes in the sample is discussed.

OVER

In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Sengs and the high amounts of contaminating host proteins. Here, we describe a workflow for the enrichment of intracellular bacteria by fluorescence activated cell sorting which in combination with highly sensitive LC-MS/MS allows monitoring of about 1200 proteins from 2 to 4 × 10. internalized bacterial cells as starting material.

mitral-valve

意外的成功

https://doi.org/10.1007/978-1-4939-8695-8Subcellular fractionation; Protein quantification; Metaproteomics; Microbes; Proteome analysis; Post-tran

Anticoagulants

Dörte BecherIncludes cutting-edge methods and protocols for the study of microbial proteomics.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the

capillaries

Eeg332

Next-Generation Trapping of Protease Substrates by Label-Free Proteomicsd, concentrated by an 1D-short gel, and identified by LC-coupled mass spectrometry (LC-MS) followed by label-free quantification. The identification of four known FtsH substrates validated this approach and suggests that it is generally applicable to AAA. proteases.