Titlebook: Microbial Lipid Production; Methods and Protocol Venkatesh Balan Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20

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Quantification of Lipid Content in Oleaginous Biomass Using Thermogravimetry,esidue under inert atmosphere, whereas other constituents of oleaginous biomass (such as proteins and carbohydrates) primarily degrade below 350 °C. As a result, triglyceride content of biomass can be estimated using TG by determining the mass loss of the sample in the temperature interval of 370–450 °C.

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Extraction and Characterization of Lipids from Macroalgae,ction of lipids in photosynthetic cells/tissues and therefore do not provide an accurate accounting of lipid production. Here we present a 2-EE lipid extraction protocol, a method which has been demonstrated to be superior to conventional lipid extraction methods for microalgae, adapted for use with macroalgae.

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Genetic Engineering Approaches Used to Increase Lipid Production and Alter Lipid Profile in Microbetegies at the direction of increased supply of substrates, regulation of lipid synthesis pathway, regulation of lipid catabolic pathway, and regulation of lipid profiles are described. These methods provide promising insights to promote the optimization of lipid accumulation and properties.

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Extraction Methods Used to Separate Lipids from Microbes,upercritical CO. extraction), chemical treatment (acid or base, enzymes), mechanical operations (such as sonication and ball beating), liquid–solid separation, liquid–liquid extraction, and gravimetric or chromatographic analysis.

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Alkaline and Alkaline-Oxidative Pretreatment and Hydrolysis of Herbaceous Biomass for Growth of Olend xylose as well as acetate and phenolic monomers that may all serve as a source of renewable carbon to produce microbial lipids. Application of these mild pretreatment conditions minimizes the generation of inhibitors, enabling microbial cultivations to often be performed without the need for detoxification.

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