Titlebook: Microbial Biofilms; Methods and Protocol Gianfranco Donelli Book 2014 Springer Science+Business Media New York 2014 assay protocols.bacteri

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of ongoing European and international projects are highligh.Accessing remote instrumentation worldwide is one of the goals of e-Science. The task of enabling the execution of complex experiments that involve the use of distributed scientific instruments must be supported by a number of different ar

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Aqueous Two-Phase System Technology for Patterning Bacterial Communities and Biofilmsd allows us to culture submillimeter-sized bacterial communities in spatially distinct spots while maintaining a degree of chemical connectedness to each other through the aqueous phase. Given sufficient time, these bacterial cultures develop biofilms, each corresponding to the footprint of the drop

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Advanced Techniques for In Situ Analysis of the Biofilm Matrix (Structure, Composition, Dynamics) byprovide guidance on how to examine the various extracellular compartments in between microbial cells and communities associated with interfaces. The current options for fluorescence staining of matrix compounds and extracellular microhabitats are presented. Furthermore, practical aspects are discuss

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Experimental Approaches to Investigating the Vaginal Biofilm Microbiomeproaches for studying vaginal microbial biofilm samples with a focus on specimen preparation for subsequent analysis. The techniques include fluorescence microscopy, fluorescence in situ hybridization, and scanning and transmission electron microscopy. Isolation of microbial DNA and RNA from these s

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Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopedic Infections number of primary surgeries translates to tens of thousands of revisions due to prosthetic joint infection (PJI). In many cases the only solution is revision surgery in which the hardware is removed. This process is extremely long and painful for patients and is a considerable financial burden for

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Animal Models to Evaluate Bacterial Biofilm Developmentthogenesis of medical biofilms. In addition, animal models are an essential tool in testing the hypothesis generated from clinical observations in patients and preclinical testing of agents showing in vitro antibiofilm effect. Here, we describe three animal models — two non-foreign body . biofilm mo