Titlebook: Microarrays in Clinical Diagnostics; Thomas O. Joos,Paolo Fortina Book 2005 Humana Press 2005 DNA.PCR.SNP.diagnostics.genes.hybridization

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Noncontact Laser Microdissection and Pressure Catapulting,reas from object slides or from living cell culture down to a resolution of individual single cells and subcellular components like organelles or chromosomes, respectively. After microdissection this sample is directly catapulted into an appropriate collection device. As the entire process works wit

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Applications of the Universal DNA Microarray in Molecular Medicine,conventional hybridization-only arrays. The intent of this article is to provide investigators with a perspective on current uses of this approach, as well as to serve as a practical guide to implementation.

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,Molecular Diagnostic Testing for Inherited Thrombophilia Using Invader®,bin G20210A was the non-PCR-based method called Invader®, developed by Third Wave Technologies. The remainder of the clinical laboratories reported testing for these variants using PCR-restriction fragment length polymorphism (RFLP), allele-specific PCR, and allele-specific hybridization. Emerging m

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Controlled Agitation During Hybridization,overcome the diffusion limit in microarray hybridization experiments. Surface acoustic waves on a piezoelectric substrate are coupled with the sample fluid on a microarray, where they act as a highly efficient agitation source. We demonstrate that the diffusion limit can be overcome in this fashion,

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Multiplexed Cytokine Sandwich Immunoassays Clinical Applications,nd compare multiple cytokines simultaneously in human serum. Serum poses a challenge for multiplexed cytokine analysis owing to the presence of numerous proteins and other potentially interfering factors. Assessment of the “real world” performance of the multiplexed, microsphere-based flow cytometry

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Validation and Quality Control of Protein Microarray-Based Analytical Methods,cate analysis, array orientation control features, on-array controls, normalization) have been implemented to control and assess analytical factors that might compromise data generated from a microarray. The current range of measures taken to ensure the analytical accuracy and quality of data genera

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ially convicted in a court of law.) Allegations in both the mainstream and social media allege that the prophet has been involved in clandestine sexual encounters, including rape of congregants which are all, one way or the other, swept under the carpet on the auspices of the dictum “touch not the a