Titlebook: Microarrays; Volume I: Synthesis Jang B. Rampal Book 2007Latest edition Humana Press 2007 Biochip.DNA.Ligation.Microarray.Nucleotide.PAC.S

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Introduction, preparation of arrays can be performed either by . synthesis of biomacromolecules on solid substrates or by spotting of . synthesized biomacromolecules onto the substrate surface. The application of microarrays includes spatial addressing with target (macro) molecules and screening for interactions

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Current Microarray Surface Chemistries, and the solid support. Factors such as probe loading, spot morphology, and signal-to-noise ratio are all intimately linked to surface chemistry. Surface chemistry also significantly impacts important performance parameters such as three-dimensional structure of the immobilized biomolecules and nons

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Nonfouling Surfaces,e.g., nucleic acid or antibody) and its target in the sample milieu to produce an assay signal specific to analyte. These interfacial interactions produce the assay result with the associated assay requirements for sensitivity, specificity, reproducibility, and ease-of-use. For surface-capture assay

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Optimization of Oligonucleotide DNA Microarrays,arget DNA with high sensitivity and selectivity. Parameters determining the performance of the microarray are spot morphology, probe and target density, background, and selectivity. More than 10 variables can be adjusted to obtain a well-optimized protocol. However, some variables only affect spot m

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Evaluating the Quality of Data From Microarray Measurements, obstacle for realizing its potentials. In the present study 60-mers oligonucleotide target immobilized on coated glass slides were utilized as a model system to investigate parameters, such as target concentration, retention, signal linearity, and fluorescence properties of fluorophores, which like

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Choice of Polymer Matrix, Its Functionalization and Estimation of Functional Group Density for Prepy simultaneously a large number of targets in a given sample. Postsynthesis immobilization, the most widely used method, involves the noncovalent and covalent fixing of suitably modified oligonucleotides on the solid supports. Among the various functional groups aminoalkyl, hydroxyalkyl, mercaptoalk

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