Titlebook: MicroRNA Protocols; Shao-Yao Ying Book 2013Latest edition Springer Science+Business Media, LLC 2013 Drug agent development.Expression prof

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Identify Intronic MicroRNA with Bioinformatics,n developed to identify novel miRNA. In human miRNA study, many studies search for the mostly complete human genomic sequence. Here, we report a rapid bioinformatics approach to mine miRNA from gene specific introns. Intronic miRNA may directly regulate the expression of its target genes during deve

Fabric

Cloning, Expression, and Functional Analysis of Genomic miRNA Using Retroviral System in Cancer Celaintain the necessary motifs of native enhancer for enhancement of mature miRNA expression. Furthermore, the procedure eliminates miRNA extraction and cDNA synthesis before cloning and sequential cloning of more than one miRNA makes this protocol cost- and time-effective to eliminate many frequent c

Neuropeptides

miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing, this chapter the protocols for cloning artificial and natural miRNAs (or shRNAs), evaluating their efficiency in downregulating gene expression, and also discuss the potential applications of these vectors.

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Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs,est in clinical and circumstantial malfunction; for example, type 2 myotonic dystrophy and fragile X syndrome were found to be associated with miRNAs derived from introns. Intronic miRNA is a new class of miRNAs derived from the processing of nonprotein-coding regions of gene transcripts. The intron

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Botao Zhao,Bing Huang,Wei Li,Youxin Jinware that safety is an issue. Therefore, there is a growing evidence for encouraging greater understanding of safety and reliability requirements issues, right 978-1-4757-6399-7978-0-387-35097-4Series ISSN 1868-4238 Series E-ISSN 1868-422X

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1064-3745 ghly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible labora978-1-4939-6261-7978-1-62703-083-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer,d RNA and visualization of Dicer cleavage products by autoradiography. The point-by-point steps of substrate preparation, recombinant Dicer cleavage assay, and northern blotting are described in this manuscript.