书目名称 | MicroRNA Protocols | 编辑 | Shao-Yao Ying | 视频video | | 概述 | Fully updates and expands upon the successful miRNA methodologies of the first edition.Features potential applications in cosmetics, wound healing, and prostate cancer.Includes key tips and implementa | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Extensive studies have been conducted on the identification, biogenesis, and processing of microRNA (miRNA) as well as research on the exact mechanism by which miRNAs bring about translational silencing of their targets. In addition, numerous publications point to an important role of miRNAs in development, reprogramming, epigenetics, pathogenesis of cancer, oncogenes and tumor suppressors, biomarkers of various disease onset, and regulation of adipogenesis and obesity; yet many questions still remain. In .MicroRNA Protocols, Second Edition., experts in the field provide up-to-date coverage of this diverse area of study. The specific chapters of this edition are related to the analysis of miRNA, targets and expression profiling, various methods to determine its regulation of gene expression, the preparation and isolation of miRNAs in specific tissues, its detection in the saliva, and potential application in cosmetics, wound healing, and prostate cancer. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible labora | 出版日期 | Book 2013Latest edition | 关键词 | Drug agent development; Expression profiling; Gene expression regulation; Target validation; miRNA; gene | 版次 | 2 | doi | https://doi.org/10.1007/978-1-62703-083-0 | isbn_softcover | 978-1-4939-6261-7 | isbn_ebook | 978-1-62703-083-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2013 |
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