Titlebook: MicroRNA Protocols; Shao-Yao Ying Book 2006 Humana Press 2006 Vivo.evolution.genes.hybridization.miRNAs.tissue

barium-study

漂泊

Structure Analysis of MicroRNA Precursors, analysis with the use of various nucleases and metal ions is performed. The presented protocols include the design of DNA template-phage promoter fusions to generate natural precursor ends, and the tests performed to check the sequence and structure homogeneity of the in vitro transcripts prior to probing their structures.

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Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiationpoietic stem/progenitor cells. We discuss in vitro and in vivo functional assays that can be used to examine the roles of miRNAs during hematopoietic lineage differentiation. The methods and principles described here should also be applicable to study the roles of miRNAs in the differentiation and function of nonhematopoietic cell types.

枫树

miRBase:The ,,scoveries, analyze genomic coordinates and context, and mine relationships between miRNA sequences. miRBase also provides a confidential gene-naming service, assigning official miRNA names to novel genes before their publication. The methods outlined in this chapter describe these functions. miRBase is freely available to all at ..

conflate

jealousy

Cloning MicroRNAs From Mammalian Tissues,ion of adapter oligonucleotides to each terminus allows amplification using reverse transcription (RT)-polymerase chain reaction (PCR). Plasmid cloning of multiple miRNA sequences and subsequent DNA sequence analysis enable both bioinformatic characterization of the various miRNAs and experimental vali-dation of their accumulation in cells.

大洪水

BOAST

Book 2006h the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.

显而易见

1064-3745 arch community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.978-1-61737-656-6978-1-59745-123-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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