Titlebook: MicroRNA Profiling; Methods and Protocol Sweta Rani Book 2017 Springer Science+Business Media New York 2017 human diseases.cell viability a

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MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells,RNA (miRNA) and their target genes following a two-step RT-PCR miRNA profiling method in bronchial cell specimens from CF and control individuals where 667 human miRNA were examined. We also describe an approach to experimental modulation of these miRNA in vitro.

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A Multiplex Ligation Assay for miRNA Copy Number Profiling,in the coding ligation these templates are used to generate, linearly amplified, DNA products encoded by length. The resultant number of each DNA product is proportional to the original number of miRNA molecules. The products and their corresponding miRNA can be identified and quantified using common DNA sizing methods such as electrophoresis.

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Duncan Kilburn,Yunke Song,Tza-Huei Wang,Kelvin J. Liu