Titlebook: MicroRNA Detection and Target Identification; Methods and Protocol Tamas Dalmay Book 2023Latest edition The Editor(s) (if applicable) and T

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1064-3745 expertsThis updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRN

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1064-3745 nd Target Identification: Methods and Protocols, Second Edition. aimsto ensure successful results in the further study of this vital field..978-1-0716-2984-0978-1-0716-2982-6Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Cristina Gómez-Martín,Ernesto Aparicio-Puerta,Michael Hackenberg

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K. M. Taufiqul Arif,Rachel K. Okolicsanyi,Larisa M. Haupt,Lyn R. Griffiths

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Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine,cellular carcinomas or in case of liver cirrhosis..Here, we provide a protocol for an expression profiling study based on qPCR analyses aimed at finding novel candidate miRNAs via small-scale or large-scale screening and evaluation experiments using liquid biopsies of blood plasma, ascites, and urin

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sRNAtoolbox: Dockerized Analysis of Small RNA Sequencing Data in Model and Non-model Species,cts like the preparation of the local database, expression profiling, or differential expression analysis as well as more advanced features such as quantification of exogenous RNA content and data analysis in non-model species.

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Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering,irected replacement of candidate MREs with molecular barcodes at endogenous loci. Subsequently, the effect of MRE mutation on transcript abundance (i.e., MRE activity) can be rapidly evaluated by routine quantitative PCR. This strategy enables functional investigation of a putative miRNA-target pair