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Titlebook: MicroRNA Detection and Target Identification; Methods and Protocol Tamas Dalmay Book 2023Latest edition The Editor(s) (if applicable) and T

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书目名称MicroRNA Detection and Target Identification
副标题Methods and Protocol
编辑Tamas Dalmay
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: MicroRNA Detection and Target Identification; Methods and Protocol Tamas Dalmay Book 2023Latest edition The Editor(s) (if applicable) and T
描述This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe. in silico .analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition. aimsto ensure successful results in the further study of this vital field..
出版日期Book 2023Latest edition
关键词Target validation; miRNAs; Detection methods; Expression level; In silico analysis
版次2
doihttps://doi.org/10.1007/978-1-0716-2982-6
isbn_softcover978-1-0716-2984-0
isbn_ebook978-1-0716-2982-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Detection of miRNAs, each with their own strengths and weaknesses. Some of these techniques have been combined to detect miRNAs as disease biomarkers in point-of-care (POC) settings. Nonetheless, there is still potential for further innovation to facilitate the detection of miRNAs.
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Determining miRNA Expression Patterns in Xenopus, gene expression. In this chapter, we describe how to visualize miRNAs in . or . by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.
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