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Titlebook: Methods of Protein Separation; Nicholas Catsimpoolas Book 1976 Springer Science+Business Media New York 1976 Filtration.biological.biology

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发表于 2025-3-21 19:13:35 | 显示全部楼层 |阅读模式
书目名称Methods of Protein Separation
编辑Nicholas Catsimpoolas
视频video
丛书名称Biological Separations
图书封面Titlebook: Methods of Protein Separation;  Nicholas Catsimpoolas Book 1976 Springer Science+Business Media New York 1976 Filtration.biological.biology
描述This open-end treatise on methods concerning pro tein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles för achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaining insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection and/or modification of existing methodology to carry out the desired separation. In such cases, the gained experience provi
出版日期Book 1976
关键词Filtration; biological; biology; development; enzyme; enzymes; experiment; protein; proteins; society; tissue;
版次1
doihttps://doi.org/10.1007/978-1-4684-9984-1
isbn_softcover978-1-4684-9986-5
isbn_ebook978-1-4684-9984-1
copyrightSpringer Science+Business Media New York 1976
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Analytical and Preparative Polyacrylamide Gel Electrophoresis,se species first in two-component systems, then in ever more complex systems and finally to reconstitute life as the sum of its component chemical reactions. The first task, i.e., the isolation of nature’s building-block molecules, remains a formidable task. This chapter is concerned with the instru
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Estimation of Molecular Weight by Gel Filtration and Gel Electrophoresis,tein molecular weights. However, the precision and accuracy of these methods have never been rigorously evaluated by formal statistical methods. In contrast, a detailed objective statistical analysis has been applied to PAGE in the absence of SDS (Rodbard and Chrambach, 1970, 1974). This statistical
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Zone Electrophoresis, Isoelectric Focusing, and Displacement Electrophoresis (Isotachophoresis) in of some more-or-less rigid supporting medium, the presence of which can, however, have several undesirable consequences: (1) The solute of interest is adsorbed, leading to low recoveries and (partial) loss of biological activity. (2) Cells and many other particles are too large to penetrate electrop
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Hydrophobic Adsorption Chromatography of Proteins,s a parameter for nonspecific adsorption chromatography of proteins. One of the reasons for the delay of investigations of possible hydrophobic effects probably was the assumption that hydrophobic groups of proteins generally are situated in the interior of the native protein molecule and thus are i
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Sievorptive Chromatography,s ability provides a powerful incentive to continually improve the procedures for the isolation of macromolecules. The scope of biological separations is overwhelmingly diverse. Even when the discussion is restricted to proteins, the problems range from the separation of macromolecules that strongly
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