Titlebook: Methods of Hybridoma Formation; Arie H. Bartal,Yashar Hirshaut Book 1987 The Humana Press Inc. 1987 DNA.Expression.Interferon.Macrophages.

Callus

侵害

教唆

Culture Methods for the Selection and Isolation of Stable Antibody-Producing Murine Hybridomas,selection and expansion of fusion cultures. The later procedure provides for reliable estimation of the stability of hybrid clones and ensures the identification of hybridomas that continue to secrete antibody.

overreach

instructive

Statistical Assessment of Hybridoma Monoclonality After Subcloning by the Limiting Dilution Techniqlishing the monoclonality of their hybridomas. Although the application of the Poisson distribution for statistically analyzing a single subcloning has been described in great detail (2,3), in hybridoma studies it is customary to subclone at least twice and the statistics for repetitive subclonings

参考书目

In Vitro Immunization for the Generation of Hybridomas Using Serum-Free Medium,operties. One approach to the improvement of hybridoma formation techniques is to attempt to define the components and reaction conditions that result in maximal formation of antibody-secreting hybridomas. Until recently, all aspects of in vitro immunization and hybridoma formation have involved an

Dorsal

Human Hybridomas,es secreting human antibody. However, the loss of human chromosomes from mouse x human hybridomas is not random. It is known, for example, that human chromosomes 14 and 22, which bear the Ig heavy chain and λ light chain locus, respectively, are preferentially retained, whereas chromosome 2, bearing

Confound

Antibody-Secreting Human-Human Hybridomas,lone in semisolid or liquid medium, and they frequently lose their Ig secretion after a few months in culture (3,6). Considerable efforts have therefore been invested to develop improved malignant fusion partners.

macrophage

Conflagration