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Titlebook: Methods in Mammary Gland Biology and Breast Cancer Research; Margot M. Ip,Bonnie B. Asch Book 2000 Springer Science+Business Media New Yor

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书目名称Methods in Mammary Gland Biology and Breast Cancer Research
编辑Margot M. Ip,Bonnie B. Asch
视频video
图书封面Titlebook: Methods in Mammary Gland Biology and Breast Cancer Research;  Margot M. Ip,Bonnie B. Asch Book 2000 Springer Science+Business Media New Yor
描述approaches to the experimental problems that still face us in understanding this most fascinating of organs. Too many people contributed to the completion of this volume to allow acknowledg­ ment of all the individual efforts, but we particularly thank the reviewers whose input into the editorial process was invaluable and the authors of these chapters who revised their text, sometimes more than once, to bring it to the high standards set by the Editors. The Com­ mittee gratefully acknowledges the support ofVysis, Inc. , in the publication of a color figure in Chapter 19, by S. Weber-Hall and Trevor Dale. Finally, we wish to express our heartfelt appreciation to Margot Ip and Bonnie Asch, who worked long and hard to bring this volume to fruition. Margaret C. Neville for the Committee on Mammary Gland Biology Preface One of the most exciting and beneficial developments in research on mammary gland biology and breast cancer has been the influx of increased funding to support this work. This influx, which has been due primarily to the tireless efforts of breast cancer activists to gamer addi­ tional money from various federal and state sources, has led to a rapid expansion of research
出版日期Book 2000
关键词carcinogenesis; carcinoma; cell; cytokines; embryo; hormones; metastasis; transplantation; tumorigenesis
版次1
doihttps://doi.org/10.1007/978-1-4615-4295-7
isbn_softcover978-1-4613-6927-1
isbn_ebook978-1-4615-4295-7
copyrightSpringer Science+Business Media New York 2000
The information of publication is updating

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A Comparison of the Salient Features of Mouse, Rat, and Human Mammary Tumorigenesisls in the literature but to emphasize key features of the main models. The information presented here is very much the authors’ interpretations of selected data in the literature, which, hopefully, will stimulate spirited discussion and new ideas.
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Xenograft Models of Human Breast Cancer Lines and of the MCF10AT Model of Human Premalignant, Prolif breast epithelial cell lines occurs in xenografts, providing a model to study early events in human breast disease. Tumorigenicity of breast cancer cell lines may be enhanced either by suspending cells in Matrigel prior to injection into the subcutis or by injecting the cells into a mammary fat pad
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Implantation and Characterization of Human Breast Carcinomas in SCID Mice of human breast carcinomas in SCID mice, using the large abdominal (gonadal) fat pad as an implantation site. Previously, we have shown that 12 (25%) out of 48 xenografts grew and reached a size of 1–2 cm within 2–6 months; these tumors were then sequentially passaged in SCID mice. In addition, we
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The Cleared Mammary Fat Pad and the Transplantation of Mammary Gland Morphological Structures and Ceastic, or malignant lesions of the mammary gland into syngeneic hosts. The cleared fat pad retains the microenvironment necessary for the normal morphological growth of transplanted mammary gland elements. The biological development of mammary gland structures can be evaluated and lesions can be tes
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Slow-Release Plastic Pellets (Elvax) for Localized , Treatments of Mouse and Rat Mammary Tissueow-release implant technique. Elvax40P. is an ethylene vinyl acetate copolymer. Formed into a matrix containing test materials such as hormones or growth factors, Elvax releases metered amounts and can be used to treat small zones within the mammary gland. Importantly, Elvax is generally nondenaturi
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Collagen Gel Method for the Primary Culture of Mouse Mammary Epithelium “ductlike” morphogenesis, and hormonal responsiveness. Subcutaneous mouse mammary glands are harvested, digested with collagenase, and epithelial cells obtained after Percoll gradient centrifugation. MECs are then mixed with neutralized, isosmotic collagen, which is pipetted into culture dishes and
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