| 书目名称 | Methods in Cellular Imaging | | 编辑 | Ammasi Periasamy (Director, Professor of Biology a | | 视频video | | | 概述 | Well illustrated book explains basic concepts and imaging procedures.Authors descrube approaches to selecting epiflourescence microscopy, detectors, and image acquisition and processing software for d | | 丛书名称 | Methods in Physiology | | 图书封面 |  | | 描述 | Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The aut | | 出版日期 | Book 2001 | | 关键词 | Cellular Imaging; biology; biophysics; cell; cell biology; gene; gene expression; membrane; microscopy; prote | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4614-7513-2 | | isbn_ebook | 978-1-4614-7513-2Series ISSN 2628-7471 Series E-ISSN 2628-748X | | issn_series | 2628-7471 | | copyright | American Physiological Society 2001 |
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发表于 2025-3-21 17:39:09
