Titlebook: Metastasis; Methods and Protocol Ulrike S. Stein Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 NCI60 tumor c

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In Vitro 3D Models of Tunable Stiffnessan in vitro setting. Use of such a model provides a robust surrogate for in vivo testing, enabling large-scale interrogation into the effect of certain treatment conditions. This adapted protocol describes a high throughput and readily accessible composite alginate hydrogel system for spheroid forma

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Preparation and Culture of Organotypic Hippocampal Slices for the Analysis of Brain Metastasis and P setups that faithfully mimic these processes are necessary to understand the mechanism of brain metastasis and to develop new improved therapeutic strategies. Here, we describe an in vitro model, which closely resembles the in vivo situation. Organotypic hippocampal brain slice cultures (OHSCs) pre

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Probing Intravascular Adhesion and Extravasation of Tumor Cells with Microfluidicsrculating tumor cells (CTCs) are transported by body fluids to reach distant organs, where they will extravasate and either remain dormant or form new tumor foci. Development of methods to study the behavior of CTCs at the late stages of the intravascular journey is thus required to dissect the mole

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Real-Time Cell Migration Monitoring to Analyze Drug Synergism in the Scratch Assay Using the IncuCyt to identify drugs, which inhibit cell migration as one feature for metastatic potential of cancer cells. One such migration assay is the wound healing or scratch assay, designed to allow cells for closure of an artificially generated gap (wound/scratch) in the monolayer. To identify possibly effect

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Matrix Degradation Assay to Measure the Ability of Tumor Cells to Degrade Extracellular Matrixorder to invade the surrounding stroma and intravasate into the circulatory system. In this chapter, we describe the 2D-fluorescent matrix degradation assay, a highly sensitive and reproducible in vitro method used to measure invadopodia-mediated ECM degradation. We provide a detailed protocol on ho

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Evaluation of Real-Time In Vitro Invasive Phenotypesrated under these standardized conditions are reproducible and characteristic of individual tumor cell lines. The complex kinetic features of the data can be analyzed using parameters modeled after pharmacokinetic data processing. Application of the method to the array of tumor types included in the