Titlebook: Metagenomics; Methods and Protocol Wolfgang R. Streit,Rolf Daniel Book 2023Latest edition The Editor(s) (if applicable) and The Author(s),

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Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Unicrobial community and thus do not assess the active fraction of the microbial community. Here, we describe a detailed protocol for the simultaneous extraction of DNA and RNA from marine water samples and the generation of cDNA from the isolated RNA that can be used as a universal template in various marker gene studies.

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Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resishere, carried out on metagenomic DNA gives us unique insights into the presence of AMR genes and how these are associated with mobile genetic elements that may be responsible for their dissemination and can also provide important information about the mechanisms of resistance underpinning the phenotype.

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Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities, identified by the introduction of metagenomic libraries into the organism of interest followed by screening for the desired phenotype. We present here a method to transfer metagenomic libraries from . to ., followed by screening for use of galactose as a sole carbon source.

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Isolation of a Host-Confined Phage Metagenome Allows the Detection of Phages Both Capable and Incap-associated metagenome reduces the phage diversity to a concise and accessible size. Metagenome sequencing often leads to complete genomes, and the availability of a suitable host system ensures further experimental investigation.