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Titlebook: Metabolic Profiling; Methods and Protocol Thomas O. Metz Book 2011 Springer Science+Business Media, LLC 2011 Drug analysis.Global metabolom

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发表于 2025-3-21 16:38:48 | 显示全部楼层 |阅读模式
书目名称Metabolic Profiling
副标题Methods and Protocol
编辑Thomas O. Metz
视频video
概述Illustrates the evolution of metabolic profiling from single molecule profiling to global metabolome profiling.Features cutting-edge protocols, presented from the perspective of leading experts in the
丛书名称Methods in Molecular Biology
图书封面Titlebook: Metabolic Profiling; Methods and Protocol Thomas O. Metz Book 2011 Springer Science+Business Media, LLC 2011 Drug analysis.Global metabolom
描述At the intersection of metabolite analysis, metabolic fingerprinting, and metabolomics, the study of metabolic profiling has evolved steadily over the course of time as have the methods and technologies involved in its study.In Metabolic Profiling: Methods and Protocols, expert researchers in the field present protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling.Comprised of the most essential techniques, this volume covers topics from inborn errors of metabolism and drug metabolite analysis to nuclear magnetic resonance metabolic profiles.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Metabolic Profiling: Methods and Protocols serves as a resource for both established and new investigators in this vital and ever-developing field.
出版日期Book 2011
关键词Drug analysis; Global metabolome profiling; Inborn errors; Metabolic fingerprinting; Metabolomics; Single
版次1
doihttps://doi.org/10.1007/978-1-61737-985-7
isbn_softcover978-1-4939-5816-0
isbn_ebook978-1-61737-985-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2011
The information of publication is updating

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发表于 2025-3-21 23:13:44 | 显示全部楼层
Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism,ly in blood and urine. For years, the most widespread technique in use was ion-exchange chromatography followed by post-column derivatization with ninhydrin, a method which is the basis of numerous automated AA analyzers with a throughput of about eight samples/day. The emergence of tandem mass spec
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Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry, of metabolic disorder accumulate disease-specific acylcarnitines that correlate with the acyl coenzyme A compounds in the affected mitochondrial metabolic pathways. For example, propionylcarnitine accumulates in patients with both propionic and methylmalonic acidemias. The test identifies and quant
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,HPLC Analysis for the Clinical–Biochemical Diagnosis of Inborn Errors of Metabolism of Purines and ates that induce transient or permanent alterations of metabolism. In particular, the diagnosis of several inborn errors of metabolism (IEMs) is accomplished by the analysis of circulating and excreted purines and pyrimidines. It is certainly advantageous to simultaneously determine the full purine
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Bile Acid Analysis in Various Biological Samples Using Ultra Performance Liquid Chromatography/Elecd exact BA behavior, the precise distribution of BAs in vivo must be determined. However, to date, it has been difficult to know the precise roles of BA due to variations in BA molecules including conjugated forms. Thus, we reconsidered BA extraction methodology and introduced an ultra performance l
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Analysis of Glycolytic Intermediates with Ion Chromatography- and Gas Chromatography-Mass Spectrome gas chromatography (GC)-MS as complementary methods. With IC-MS-MS, pyruvate, glucose-6-phosphate, fructuse-6-phosphate, fructose-1,6-bisphosphate, phosphoenolpyruvate, and the sum of 2-phosphoglyceraldehyde + 3-phosphoglyceraldehyde can be quantified. With GC-MS using selected ion monitoring, glyc
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Analysis of the Citric Acid Cycle Intermediates Using Gas Chromatography-Mass Spectrometry,ntly related and unrelated pathways of metabolism. Relationships of the CAC intermediates, as measured by their concentrations and relative ratios, offer useful information to understanding interrelationships between the CAC and metabolic pathways under various physiological and pathological conditi
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High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)-Based Drug Metabolite Profiling,overy and development. Mass spectrometry, particularly when combined with high-performance liquid chromatography (HPLC-MS), can enable detailed structural information to be obtained on the metabolites of a drug or xenobiotic as a result of metabolism. The successful identification of drug metabolite
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