Titlebook: Metabolic Flux Analysis; Methods and Protocol Jens O. Krömer,Lars K. Nielsen,Lars M. Blank Book 2014 Springer Science+Business Media New Yo

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Correction of MS Data for Naturally Occurring Isotopes in Isotope Labelling Experimentss. Quantitative applications—e.g., .C metabolic flux analysis—require correction of raw MS data (isotopic clusters) for the contribution of all naturally abundant isotopes. This chapter describes how to perform such correction using the software IsoCor. This flexible, user-friendly software can be u

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Steady-State 13C Fluxomics Using OpenFLUXsumption/production rates. However, .C-MFA is computationally more demanding. A nonlinear least-square optimization process is employed to constrain metabolic fluxes using atom balance models and experimentally measured .C labelling pattern of intracellular or proteinogenic metabolites. OpenFLUX was

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Book 2014but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell. Divided into convenient thematic parts, topics in this essential volume include the fundamental characteris

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