书目名称 | Metabolic Flux Analysis |
副标题 | Methods and Protocol |
编辑 | Jens O. Krömer,Lars K. Nielsen,Lars M. Blank |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .Metabolic Flux Analysis: Methods and Protocols .opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell. Divided into convenient thematic parts, topics in this essential volume include the fundamental characteristics of the underlying networks, the application of quantitative metabolite data and thermodynamic principles to constrain the solution space for flux balance analysis (FBA), the experimental toolbox to conduct different types of flux analysis experiments, the processing of data from .13.C experiments and three chapters that summarize some recent key findings. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Metabolic Flux Analysis.: Methods and Protocols. presents protocols |
出版日期 | Book 2014 |
关键词 | cell culture; flux analysis; metabolomics; stoichiometry; thermodynamics |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-1170-7 |
isbn_softcover | 978-1-4939-4159-9 |
isbn_ebook | 978-1-4939-1170-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2014 |